I tata nei, kua ahu whakamua te roopu hangarau koiora o te pukohu me te pukohu i te Institute of Oceanology o te Hainamana Academy of Sciences ki te whakawhanake i nga rauemi astaxanthin mai i te chlorella whero. I kitea he maha -korero e pa ana ki te whakaahua, penei i te anaerobic respiratory glycolysis (EMP), aerobic respiratory tricarboxylic acid cycle (TCA), pentose phosphate pathway (PPP) me te mitochondrial respiration alternate oxidase pathway (AOX), he nui nga paanga ture ki te whakahiato me te whakaemi astaxanthin. I whakaputaina nga hua i roto i nga pepa rangahau e toru i roto i te Hangarau Hangarau.
He whero kanapa a Astaxanthin, he tino kaha te tae, he kaha antioxidant me te maha o nga mahi koiora, a he whanui te tono mo te kai me te kai hauora, rongoa me nga whakapaipai.Ko te Erythrochlorella he momo-kotahi puohu kaakaariki e whai kiko ana ki te astaxanthin, a koinei hoki te tino rauemi koiora hei whakaputa i te astaxanthin taiao i te ao. I whakaponohia i mua ko te astaxanthin biosynthesis te nuinga i peia e te kaha o te marama me te whakaawe i te korenga matūkai. Ko te nuinga o nga rangahau i te kainga, i waho hoki i aro ki nga tukanga anabolic e whakawhirinaki ana ki te whakaahua (pēnei i te whakaahua, te whakamarumaru whakaahua me te tipu o te pūtau), he iti noa te aro ki te pirau me te manawa pau. Ahakoa i roto i nga tau tata nei, ko te whakahiatotanga whakaahua anake me te rewharewha chloroplast i whai waahi, engari ko te mahi a te -kore-whakaahua-whakaahuatanga o te huarahi rewharewha rewharewha i roto i te whakahiato astaxanthin me te whakaemi i roto i te S. novosa kaore i tino korerotia.
I kitea, i whakapumautia e te roopu he maha nga ara-whakaahua kore-whakaahua ka taea te whakahaere i te whakahiato me te whakaemi astaxanthin. I kitea e te rangahau ko etahi atu huarahi oxidase o te mahi manawa mitochondrial me te whero kua kitea ko te whakaemi astaxanthin he hononga kino, i muri i te aukati i te huarahi e whakaarohia ana, e tata ana ki te manawa i waenganui o te waikawa pyruvic metabolite me te glyceraldehyde 3 phosphoric acid ihirangi i tino pai ake, i te wa ano e whakatairanga ana i te whakatipuranga o nga momo, te NADPH, te whakatairanga i te whakangao o nga momo, te whakangao o nga momo, te NADPH. Ka taea te whakatairanga astaxanthin synthesis. Ko te whakaaro ko te whakaritenga o te manawa ka taea te whakatairanga i te koiora astaxanthin me te whakaemi.
I tua atu, i whakapumau ano matou ka taea e te huringa TCA, te pokapū o te whakangao hiko i roto i te manawa -kore e whakawhirinaki ana ki te whakaahua, ka taea te hanga anga waro mo te whakahiato astaxanthin me te whakatairanga i te whakaemi astaxanthin ki roto i nga pūtau ma te taapiri i nga metabolites takawaenga o te manawa. Ko tana tikanga whakahaere e whai ake nei: Ko nga metabolites takawaenga ka taea e te waikawa fumaric te whakapai ake i te rewharewha o te EMP, TCA me te PPP e toru nga huarahi matua, ka nui ake te waikawa pyruvic me te glyceraldehyde 3 waikawa phosphoric, i tetahi taha, ma te whakatairanga i te astaxanthin synthesis precursor o te isoamyl ene pyronthesis, te whakatairanga tika i te ringa o te pyruvic me te glyceraldehyde 3. te whakahiatotanga o nga waikawa ngako, na reira ka whakatere i te mahi astaxanthin esterification, te whakatairanga autaki i te whakahiato astaxanthin. Ko te Oxaloacetate, tetahi atu metabolite o te huringa TCA, ka taea te whakatairanga tika i te whakahiato astaxanthin ma te whakanui ake i te taputapu me nga taumata NADPH. Ka whakatauritea ki te waikawa fumaric, he tere ake, he nui ake te painga o te waikawa oxaloacetic ki te whakatairanga, na te mea he pai ake te mahi a te transmembrane.
Ko nga hua ka whakapai ake i te punaha whanaketanga tauine astaxanthin whero o te ariā taketake me te hangarau whanaketanga, ka awhina i te whakapai ake i te bioreactor i runga i nga ture huringa pūtau me nga hangarau hanga rama nui penei i te ahua whero kitea te whanaketanga, kia pai ake te mahi a nga tipu tipu tipu whakaahua, whakatairanga i te taumata whanaketanga ahumahi o te pikinga. I te wa ano, ka taea te whakamahi i nga hua rangahau hei tohutoro mo te rangahau me te whanaketanga rauemi o nga metabolites tuarua penei i te karotene o etahi atu microalgae.
