Ina tata nei, i whakaputaina e te Rangahau Horticulture i runga ipurangi he pepa rangahau ko N-glucosyltransferase GbNGT1 mai i te ginkgo e whakakii ana i te ara metabolic auxin na te kairangahau a Liu An mai i te Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences.
I kitea e te tātaritanga metabolic e 58 nga momo ginkgo me nga riaka mai i Haina me Hapani e whakarangatira nuitia ana ki te hua kapeka auxin IAA -ASP-N-glucoside, me te taumaha maroke ki runga ki te 4.13 mg/g. I tua atu, na roto i te whakamaoritanga me te tātari mahi enzyme, i kitea he N-glycosyltransferase GbNGT1 ka taea te whakakorikori IAA me IAA-ASP. Ko te Auxin te mea nui hei whakahaere i te tipu tipu. Ko tenei tuhinga e whakaatu ana i te whakarerekētanga glycosylation o te N{11}}turanga o te auxin, e whakaoti ana i te ara whakawhiti auxin.
I tirotirohia e te kaituhi e 52 nga indoles me te flavonoids, ka kitea e te enzyme he whakarereke i te turanga N o IAA-AA me IAA. Ko te whākōkī he āhuatanga o te ātete waikawa, te motuhake katote whakarewa me te tairongo teitei o te pāmahana. I kitea e te ū rāpoi me te whakahāngai i te pae-mutagenesis kua ngaro rawa atu te mutant E15A i tana kaha whakakata mo IAA-Asp me IAA. I whakamanahia ano e nga kaituhi a raatau mahi i roto i te tupeka. I te wa kotahi, whakaurua nga ira AtGH3.8 me GbNGTs (NGT kua whakarereketia ranei) kia mohio ai te koiora mai i te IAA ki te IAA-AA-N-glucoside i roto i te tupeka.
Mai i te whakaputanga tuatahi o te IAA O-glycosylation enzyme IAAGLU i roto i te Pūtaiao i te tau 1994, he maha nga hua i puta i nga kairangahau mo te paanga o te IAA O-glycosylation ki te tipu me te whanaketanga ma te whakahaere i te toenga hihiri o IAA. Heoi, ko nga rangahau o naianei mo te ara N-glycosylation me te tipu me te whanaketanga o te IAA kei te tata tonu. I kitea e tenei pepa ko te IAA o nga tipu GbNGT1 OE he iti rawa atu i tera o te momo mohoao, engari ko te tohu IAA i pa ki te pito pakiaka i tino whakanuihia; i kitea e te whakamatautau tipu pakiaka he tere ake te roa o te pakiaka o te tipu GbNGT1 nui ake i tera o te momo mohoao. Ko tenei rangahau e whakawhanui ana i te hononga i waenga i te peka IAA N-glycosylation me te tipu me te whanaketanga.
Ko Dr. Yin Qinggang, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, te kaituhi tuatahi o te pepa, ko te kairangahau a Liu An te kaituhi o te pepa. Ko nga tauira o te tohu tohu a Zhang Jing, ko Wang Shuhui nga tohu paetahi, ko Takuta Cheng Jintang i uru ki tenei rangahau. Ko Dr Sun Limin mai i te Whare Wananga Ahuwhenua o Shandong me te kairangahau a Ma Lianbao mai i te Pizhou Ginkgo Research Institute i whai waahi nui ki tenei tuhinga. I tautokohia te rangahau e te Beijing Municipal Natural Science Foundation me te National Key Research and Development Program.
